CGP77675

CGP77675 is a potent inhibitor of Src family kinases. It has been reported to inhibit phosphorylation of peptide substrates and autophosphorylation of purified Src (IC₅₀ = 5 - 20 nM and 40 nM, respectively), Src, and Src kinase family members such as LCK (IC₅₀ = 0.02 mM and 0.29 mM, respectively). It has also been shown to inhibit epidermal growth factor receptor (EGFR), Cdc2, v-Abl, and focal adhesion kinase (FAK) (IC₅₀ = 0.15 mM, > 10 mM, 0.31 mM, and 0.2 mM, respectively; Missbach et al.).


MAINTENANCE AND SELF-RENEWAL
· In combination with the GSK3 inhibitor CHIR99021 (Catalog #72052), CGP77675 is used to maintain mouse embryonic stem (ES) cells (Shimizu et al.).

· Preserves the epigenetic and genomic integrity as well as the developmental potential of ES cells (Choi et al.).