BIO
WNT pathway activator; Inhibits GSK3
概要
BIO is an indirubin compound that is cell-permeable and is a selective and potent reversible inhibitor of GSK3α and GSK3β (IC₅₀ = 5 nM) and functions as a WNT activator.
REPROGRAMMING
· Enhances reprogramming of mouse fibroblasts, neural stem cells, and thymocytes to induced pluripotent stem (iPS) cells (Lluis et al.).
MAINTENANCE AND SELF-RENEWAL
· Maintains self-renewal of mouse embryonic stem (ES) cells in the absence of LIF (Sato et al.).
· Maintains self-renewal of human ES cells in unconditioned medium through a SMAD 2/3 dependent mechanism (James et al., Sato et al.).
· Induces proliferation of neonatal and adult rat cardiomyocytes (Tseng et al.).
DIFFERENTIATION
· Promotes differentiation of cardiomyocytes from human ES and iPS cells (Lian et al.).
REPROGRAMMING
· Enhances reprogramming of mouse fibroblasts, neural stem cells, and thymocytes to induced pluripotent stem (iPS) cells (Lluis et al.).
MAINTENANCE AND SELF-RENEWAL
· Maintains self-renewal of mouse embryonic stem (ES) cells in the absence of LIF (Sato et al.).
· Maintains self-renewal of human ES cells in unconditioned medium through a SMAD 2/3 dependent mechanism (James et al., Sato et al.).
· Induces proliferation of neonatal and adult rat cardiomyocytes (Tseng et al.).
DIFFERENTIATION
· Promotes differentiation of cardiomyocytes from human ES and iPS cells (Lian et al.).
Alternative Names
GSK 3 IX; MLS 2052; 6-Bromoindirubin-3'-oxime
Cell Type
Cardiomyocytes, PSC-Derived, Pluripotent Stem Cells
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Differentiation, Expansion, Maintenance, Reprogramming
Area of Interest
Stem Cell Biology
CAS Number
667463-62-9
Chemical Formula
C₁₆H₁₀BrN₃O₂
Molecular Weight
356.2 g/mol
Purity
≥ 98%
Pathway
WNT
Target
GSK3
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | BIO | 72032, 72034 | All | English |
| Safety Data Sheet | BIO | 72032, 72034 | All | English |
数据及文献
Publications (2)
Proceedings of the National Academy of Sciences of the United States of America 2012 JUL
Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling.
Abstract
Abstract
Human pluripotent stem cells (hPSCs) offer the potential to generate large numbers of functional cardiomyocytes from clonal and patient-specific cell sources. Here we show that temporal modulation of Wnt signaling is both essential and sufficient for efficient cardiac induction in hPSCs under defined, growth factor-free conditions. shRNA knockdown of β-catenin during the initial stage of hPSC differentiation fully blocked cardiomyocyte specification, whereas glycogen synthase kinase 3 inhibition at this point enhanced cardiomyocyte generation. Furthermore, sequential treatment of hPSCs with glycogen synthase kinase 3 inhibitors followed by inducible expression of β-catenin shRNA or chemical inhibitors of Wnt signaling produced a high yield of virtually (up to 98%) pure functional human cardiomyocytes from multiple hPSC lines. The robust ability to generate functional cardiomyocytes under defined, growth factor-free conditions solely by genetic or chemically mediated manipulation of a single developmental pathway should facilitate scalable production of cardiac cells suitable for research and regenerative applications.
Chemistry & biology 2003 DEC
GSK-3-selective inhibitors derived from Tyrian purple indirubins.
Abstract
Abstract
Gastropod mollusks have been used for over 2500 years to produce the Tyrian purple" dye made famous by the Phoenicians. This dye is constituted of mixed bromine-substituted indigo and indirubin isomers. Among these�