STEMtaq™ Hot Start DNA Polymerase Master Mix Kit

STEMtaq™ Hot Start DNA Polymerase Master Mix Kit, which includes Hot Start master mix and nuclease-free water, reliably and consistently amplifies a wide range of PCR templates in a convenient master mix format that allows for room temperature setup. The enzyme is bound to a proprietary antibody that blocks activity until the initial denaturation step (94 - 95℃ for 2 minutes) of the PCR re-activates the enzyme. Hot Start PCR can in some cases eliminate or minimize primer-dimers and secondary products for some targets, and potentially improve yield. STEMtaq™ Hot Start DNA Polymerase Master Mix is a 2X concentrated, ready-to-use solution containing the Hot Start version of the STEMtaq™ DNA polymerase, dNTPs, MgCl₂, and reaction buffer. STEMtaq™ Hot Start DNA polymerase has 5’ to 3’ exonuclease activity and produces PCR fragments with a 3’ A overhang.