ArciTect™ Human HPRT Positive Control Kit

ArciTect™ Human HPRT Positive Control Kit is designed as a positive control for experiments using the ArciTect™ CRISPR-Cas9 genome editing system. The kit comprises ArciTect™ Human HPRT crRNA (2 nmol), ArciTect™ Human HPRT Primer Mix (2 nmol), ArciTect™ tracrRNA (5 nmol), and ArciTect™ Annealing Buffer (5X). ArciTect™ Human HPRT crRNA first requires annealing to ArciTect™ tracrRNA (included in the kit). This must then be combined with an ArciTect™ Cas9 Nuclease (e.g. Catalog #76002) to form a ribonucleoprotein complex. ArciTect™ Human HPRT Primer Mix can be used to amplify genomic DNA isolated from a population of transfected cells, which can subsequently be used in a T7 endonuclease I assay to determine cleavage efficiency.
This kit has been tested and validated for use with the ArciTect™ line of genome editing products. HPRT, or hypoxanthine phosphoribosyltransferase, is a housekeeping gene and a commonly used control. The kit can be used to optimize transfection protocols and act as a positive control that can be used alongside custom ArciTect™ crRNAs (e.g. Catalog #76010).