Mouse IL-5 ELISA Kit

For detection and measurement of mouse interleukin 5
概要
The Mouse Interleukin 5 (IL-5) ELISA Kit is designed for the quantitative detection and measurement of mouse IL-5 in biological fluids such as serum, plasma, and cell culture supernatants. IL-5 is produced by Th2 cells, eosinophils, and activated mast cells. IL-5 plays an important role in the recruitment, activation, proliferation, and survival of eosinophils.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Components
  • Mouse IL-5 ELISA Kit, 2 plates (Catalog #02024)
    • Mouse IL-5 ELISA Plate, 2x
    • Mouse IL-5 Standard, 1 vial
    • Mouse IL-5 Biotinylated Detection Antibody, 30 µL
    • SA-HRP, 30 µL
    • Standard Reconstitution Buffer B, 1 mL
    • Wash Buffer (20X), 120 mL
    • ELISA Diluent, 120 mL
    • SA-HRP Diluentt, 25 mL
    • TMB Substrate, 25 mL
    • Stop Solution, 25 mL
    • Adhesive Plate Covers, 6x
 
  • Mouse IL-5 ELISA Kit, 10 plates (Catalog #02025)
    • Mouse IL-5 ELISA Plate, 10x
    • Mouse IL-5 Standard, 1 vial
    • Mouse IL-5 Biotinylated Detection Antibody, 130 µL
    • SA-HRP, 130 µL
    • Standard Reconstitution Buffer B, 1 mL
    • Wash Buffer (20X), 5 x 120 mL
    • ELISA Diluent, 3 x 20 mL
    • SA-HRP Diluent, 120 mL
    • TMB Substrate, 120 mL
    • Stop Solution, 120 mL
    • Adhesive Plate Covers, 30x
Subtype
Complete Kits
Cell Type
B Cells, Granulocytes and Subsets, NK Cells, T Cells, Other Subsets
Species
Mouse
Application
ELISA
Area of Interest
Immunology
数据及文献

Data

Representative Standard Curve

• Reportable range: 3.2 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 2 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: No international standard exists for calibration.
• Recovery: A mid-curve recovery of 64 - 73% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 4.2% (CV). The inter-assay precision of this assay is 7.8% (CV).

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