Human IL-23 ELISA Kit

For detection and measurement of human interleukin 23
概要
The Human Interleukin 23 (IL-23) ELISA Kit is designed for the quantitative detection and measurement of human IL-23 in biological fluids such as serum, plasma, and cell culture supernatants. IL-23 is a heterodimeric cytokine composed of a p19 and a p40 subunit; the p40 subunit is shared with IL-12. IL-23 is secreted by activated macrophages and dendritic cells, and induces the proliferation of memory T cells and the production of IL-17.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Components
  • Human IL-23 ELISA Kit, 2 plates (Catalog #02016)
    • Human IL-23 ELISA Plate, 2x
    • Human IL-23 Standard, 1 vial
    • Human IL-23 Biotinylated Detection Antibody, 50 µL
    • SA-HRP, 30 µL
    • Standard Reconstitution Buffer B, 1 mL
    • Wash Buffer (20X), 120 mL
    • ELISA Diluent, 120 mL
    • SA-HRP Diluent, 25 mL
    • TMB Substrate, 25 mL
    • Stop Solution, 25 mL
    • Adhesive Plate Covers, 6x
 
  • Human IL-23 ELISA Kit, 10 plates (Catalog #02017)
    • Human IL-23 ELISA Plate, 10x
    • Human IL-23 Standard, 1 vial
    • Human IL-23 Biotinylated Detection Antibody, 250 µL
    • SA-HRP, 130 µL
    • Standard Reconstitution Buffer B, 1 mL
    • Wash Buffer (20X), 5 x 120 mL
    • ELISA Diluent, 3 x 120 mL
    • SA-HRP Diluent, 120 mL
    • TMB Substrate, 120 mL
    • Stop Solution, 120 mL
    • Adhesive Plate Covers, 30x
Subtype
Complete Kits
Cell Type
Dendritic Cells, Macrophages
Species
Human
Application
ELISA
Area of Interest
Immunology
数据及文献

Data

Representative Standard Curve

• Reportable range: 10 - 3160 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 4 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: No international standard exists for calibration.
• Recovery: A mid-curve recovery of 76 - 110% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 2.6% (CV). The inter-assay precision of this assay is 8.6% (CV).

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