Human Recombinant NT-4

Neurotrophin 4

概要技术资料数据及文献

概要

Neurotrophin-4 (NT-4) is a member of the nerve growth factor family which includes neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), and nerve growth factor (NGF), all of which promote the differentiation, growth, and survival of peripheral and central nervous system neurons (Eide et al.). NT-4 binds and activates tropomyosin receptor kinase B (TrkB) at the cell surface; in doing so, it acts as a survival factor for certain populations of sensory neurons (Berkemeier et al.; Skaper). It has been shown that NT-4, together with BDNF, promotes neurite extension and maturation, as well as maintenance of differentiated cerebellar granule cells (Gao et al.).

Cytokines

GLC10, GLC1O, Neutrophic factor 4, Neutrophic factor 5, Neurotrophin-5, NT-4/5, NT-5, NTF4, NTF5

Astrocytes, Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons

Human

Neuroscience, Stem Cell Biology

14.0 kDa monomer; 28.1 kDa dimer

≥ 95%

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Human Recombinant NT-4	78093, 78093.1	All	English
Safety Data Sheet	Human Recombinant NT-4	78093, 78093.1	All	English

数据及文献

Data

(A) The biological activity of Human Recombinant NT-4 was tested by its ability to promote the proliferation of a neuroblastoma cell line stably expressing TrkB (BR6). Cell proliferation was measured using a colorimetric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 5.6 ng/mL. For a representative bioactivity plot of C6 cell proliferation, contact us at techsupport@stemcell.com. (B) 1 μg of Human Recombinant NT-4 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant NT-4 is a homodimer of 14 kDa subunits with a predicted total molecular mass of 28.1 kDa.