Human Recombinant NGF-beta, ACF
Nerve growth factor-beta, animal component-free
概要
Nerve growth factor (NGF)-beta is a prototypical member of the neurotrophin family and has a role in the survival and growth of neural cells, regulating cell growth, promoting differentiation into neurons, and neuron migration. The beta subtype of NGF is biologically active in comparison to the alpha-2 and gamma-2 subtypes. NGF-beta in its secreted form can bind to tyrosine kinase A (trkA) receptor with high affinity and to p75 (NTR) with low affinity (Levi & Alemà; Sofroniew et al.). NGF has been shown to possess pro-inflammatory and pro-fibrogenic properties (Micera et al.). It has also been shown that overexpression of NGF-beta promotes differentiation of bone marrow mesenchymal stem cells into neurons through regulation of AKT and MAPK pathways (Yuan et al.). This product is animal component-free.
Subtype
Cytokines, Growth Factors
Alternative Names
Beta-nerve growth factor, beta-NGF, nerve growth factor (beta polypeptide)
Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons
Area of Interest
Immunology, Stem Cell Biology
Molecular Weight
13.6 kDa
Purity
≥ 95%
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant NGF-beta, ACF | 78151, 78151.1, 78151.2 | All | English |
| Safety Data Sheet | Human Recombinant NGF-beta, ACF | 78151, 78151.1, 78151.2 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant NGF-beta, ACF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured after 60 hours in culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 1.31 ng/mL. (B) 1 μg of Human Recombinant NGF-beta, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.