Human Recombinant IL-7

Interleukin 7

概要技术资料数据及文献

概要

Interleukin 7 (IL-7) is a member of the Type I cytokine family that is critical for T and B cell development and survival. It is produced by non-hematopoietic cells in the thymus, lymphoid organs, and by bone marrow stromal cells (Lundström et al.). IL-7 binds to a receptor (IL-7R) composed of common gamma chain and IL-7Ra (CD127) and signals through the JAK/STAT and PI3K pathways. IL-7 regulates the survival of naïve and memory CD4+ and CD8+ T cells, γδ T cells, NK T cells, innate lymphoid cells, and T regulatory cells (Carrette & Surh). Although a deficiency in IL-7R still permits the generation of normal numbers of peripheral B cells in humans, stimulation of human B cell precursors with IL-7 could promote STAT-5-dependent proliferation and survival in vitro (Clark et al.; Corfe & Paige).

Cytokines

Interleukin-7, LP-1, Lymphopoietin 1, Pre-B cell factor

Hematopoietic Stem and Progenitor Cells, Lymphocytes

Human

Immunology, Stem Cell Biology

17.5 kDa

≥ 95%

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Human Recombinant IL-7	78053, 78053.1, 78053.2, 78053.3	All	English
Safety Data Sheet	Human Recombinant IL-7	78053, 78053.1, 78053.2, 78053.3	All	English

数据及文献

Data

(A) The biological activity of Human Recombinant IL-7 was tested by its ability to promote the proliferation of 2E8 cells. Cell proliferation was measured after 66 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.06 - 0.09 ng/mL.
(B) 1 μg of Human Recombinant IL-7 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IL-7 has a predicted molecular mass of 17.5 kDa.