Human Recombinant IGF-I, ACF

Insulin-like growth factor 1, animal component-free

概要技术资料数据及文献

概要

Insulin-like growth factor 1 (IGF-I) is a polypeptide that belongs to the family of insulin-like growth factors that are similar in molecular structure to proinsulin. IGF-I binds to the IGF-I receptor and is a potent activator of the PI3K/AKT pathway and also activates ERK1/2 signaling. IGF-I is required for embryonic development, and it is produced mainly in the liver in response to a hepatocyte growth hormone. In the absence of insulin, IGF-I is necessary for the maintenance of human pluripotent stem cells (Wang et al.). Together with IL-3, IGF-I stimulates differentiation and proliferation of myeloid cells and has been shown to regulate lymphopoiesis by stimulating proliferation and differentiation of T and B cells in lymphoid organs (Heemskerk et al.). This product is animal component-free.

Cytokines, Growth Factors

IBP1, IGF-IA, IGF-IB, IGF1A, Insulin-like growth factor 1, Mechano growth factor, MGF, Somatomedin C

Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Prostate Cells

Epithelial Cell Biology, Neuroscience, Stem Cell Biology

7.7 kDa

≥ 95%

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Human Recombinant IGF-I, ACF	78142, 78142.1, 78142.2	All	English
Safety Data Sheet	Human Recombinant IGF-I, ACF	78142, 78142.1, 78142.2	All	English

数据及文献

Data

(A) The biological activity of Human Recombinant IGF-I, ACF was tested by its ability to promote the proliferation of FDC-P1 cells. Cell proliferation was measured after 72 hours in culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.856 ng/mL. (B) 1 μg of Human Recombinant IGF-I, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.