Human Recombinant HGF

Hepatocyte growth factor

概要技术资料数据及文献

概要

Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic growth factor that promotes proliferation, motility, survival, and differentiation. HGF binds and promotes the dimerization and activation of the receptor tyrosine kinase c-MET, and stimulates PI3K/AKT, FAK, JNK, and ERK1/2 signaling (Organ & Tsao). HGF stimulates migration of cells during embryogenesis, induces cell motility and scattering of epithelial cells, and regulates epithelial-mesenchymal transition. It is a chemoattractant for motor neurons, and regulates the development of sensory, sympathetic, parasympathetic, and cortical neurons as well as the proliferation of oligodendrocytes and glial development. Additionally, HGF is cytoprotective and regulates liver growth and regeneration (Nakamura et al.).

Cytokines, Growth Factors

DFNB39, F-TCF, Fibroblast-derived tumor cytotoxic factor, Hepapoietin A, HPTA, Lung fibroblast-derived mitogen, Scatter factor, SF

Airway Cells, Hepatic Cells, Intestinal Cells, Mammary Cells, Neurons, Pluripotent Stem Cells, Prostate Cells

Human

Epithelial Cell Biology, Neuroscience, Stem Cell Biology

88 - 90 kDa (single chain)

≥ 95%

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Human Recombinant HGF	78019, 78019.1, 78019.2	All	English
Safety Data Sheet 1	Human Recombinant HGF	78019, 78019.1	All	English
Safety Data Sheet 2	Human Recombinant HGF	78019, 78019.1, 78019.2	All	English

数据及文献

Data

(A) The biological activity of Human Recombinant HGF was tested by its ability to promote the proliferation of 4MBr5 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 7.2 ng/mL.
(B) 5 μg of Human Recombinant HGF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant HGF has a predicted molecular mass of 88 - 90 kDa (single chain), 59 - 61 kDa (alpha chain), or 30 - 34 kDa (beta chain).