Human Recombinant HBEGF

Heparin-binding EGF-like growth factor

概要技术资料数据及文献

概要

Heparin-binding epidermal growth factor (EGF)-like growth factor (HBEGF) is a member of the EGF family (Nishi & Klagsbrun). HBEGF promotes blastocyst adhesion to the uterine wall (Iwamoto & Mekada). It also plays a role in smooth muscle cell hyperplasia and brain injury (Nishi & Klagsburn). HBEGF produced by CD4+ T cells promotes wound healing by stimulating migration and proliferation of keratinocytes, fibroblasts, and smooth muscle cells (Blotnick et al.). It binds to EGFR, ErbB4, ErbB2, and ErbB3, activating the PI3K/AKT signaling cascade (Iwamoto & Mekada). HBEGF is produced in a variety of cells, where it contributes to physiological and pathological processes. HBEGF is overexpressed in ovarian, breast, gastric, colorectal, pancreatic, and endometrial cancers, which likely contributes to pathogenesis (Miyata et al.).

Cytokines, Growth Factors

Diphtheria toxin receptor, DTR, HBEGF, Heparin binding EGF-like growth factor

Fibroblasts, Keratinocytes, Smooth Muscle, T Cells, CD4+

Human

Epithelial Cell Biology, Immunology

9.7 kDa

≥ 95%

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Human Recombinant HBEGF	78163, 78163.1	All	English
Safety Data Sheet	Human Recombinant HBEGF	78163, 78163.1	All	English

数据及文献

Data

(A) The biological activity of Human Recombinant HBEGF was tested by its ability to promote the proliferation of 3T3 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is less than 0.75 ng/mL. (B) 2 μg of Human Recombinant HBEGF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant HBEGF has a predicted molecular mass of 9.7 kDa.