Human Recombinant GDNF, ACF

Glial cell line-derived neurotrophic factor
概要
Glial cell line-derived neurotrophic factor (GDNF) is a neurotrophic factor and a member of the tumor growth factor (TGF)-beta superfamily. The GDNF family of growth factors also includes neurturin, persephin, and artemin, which have seven conserved cysteine residues called cysteine-knot (Treanor et al.). GDNF family ligands signal through binding to specific GDNF-family receptor-α (GFRα) co-receptors and activate the RET receptor tyrosine kinase (Durbec et al.). Four different forms of GFRα co-receptors have been characterized (GFRα 1-4) out of which GDNF binds specifically to GFRα1 prior to forming a complex with RET (Airaksinen & Saarma). GDNF is known to promote survival and morphological differentiation of midbrain dopaminergic neurons in both in vivo and in vitro studies and increase their high-affinity dopamine uptake (Granholm et al.; Lin et al.). GDNF has also been shown to have restorative effects on dying dopaminergic neurons in response to degenerative toxins (Aoi et al.). GDNF, together with Human Recombinant BDNF (brain-derived neurotrophic factor; Catalog #78005), BrainPhys™ Neuronal Medium (Catalog #05790), and other supplements, can be used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into neurons (Bardy et al.). This product is animal component-free.
Subtype
Cytokines
Alternative Names
Astrocyte-derived trophic factor, ATF, ATF1, ATF2, Glial cell-derived neurotrophic factor
Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons
Area of Interest
Neuroscience, Stem Cell Biology
Molecular Weight
15.2 kDa monomer; 30.4 kDa dimer
Purity
≥ 95%
技术资料
Document Type 产品名称 Catalog # Lot # 语言
Product Information Sheet Human Recombinant GDNF, ACF 78139, 78139.1, 78139.2 All English
Safety Data Sheet Human Recombinant GDNF, ACF 78139, 78139.1, 78139.2 All English
数据及文献

Data

(A) The biological activity of Human Recombinant GDNF, ACF was tested by its ability to promote the proliferation of C6 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 1.84 μg/mL. (B) 1 μg of Human Recombinant GDNF, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

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