Human Recombinant GDF-11, ACF

Growth differentiation factor 11, animal component-free

概要技术资料数据及文献

概要

Growth differentiation factor 11 (GDF-11) is a member of transforming growth factor beta (TGF-β) family. It binds to the TGF-β receptors ALK4, ALK5, and ALK7 and activates the SMAD signaling pathway (Ho et al.). GDF-11 regulates the development of the olfactory system, retina, and pancreas, as well as anterior/posterior patterning of the axial skeleton (Lee & Lee). GDF-11 is an endocrine factor expressed in skeletal muscle, brain, and dental pulp (Kondás et al.). Studies in mice showed that GDF-11 regulates muscle and cardiac aging, and stimulates neurogenesis by remodeling blood vessels (Katsimpardi et al.; Loffredo et al.; Sinha et al.). This product is animal component-free.

Cytokines

BMP-11, Bone morphogenetic protein 11, Growth/differentiation factor 11

Neural Cells, PSC-Derived

Human

Neuroscience, Stem Cell Biology

12.5 kDa monomer; 24.9 kDa dimer

≥ 95%

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Human Recombinant GDF-11, ACF	78189, 78189.1, 78189.2	All	English
Safety Data Sheet	Human Recombinant GDF-11, ACF	78189, 78189.1, 78189.2	All	English

数据及文献

Data

(A) The biological activity of Human Recombinant GDF-11, ACF was tested by its ability to induce alkaline phosphatase activity in ATDC5 cells. Alkaline phosphatase activity was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which alkaline phosphatase activity is at 50% of maximum. The EC50 in the example above is 3.54 ng/mL. (B) 1 μg of Human Recombinant GDF-11, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant GDF-11, ACF has a predicted molecular mass of 24.9 kDa (12.5 kDa per monomer).