Human Recombinant FGF-8B, ACF
Fibroblast growth factor 8B, animal component-free
概要
Fibroblast growth factor 8B (FGF-8B) is a member of the fibroblast growth factor (FGF) family and is an isoform of FGF-8. Cytokines in the FGF family possess broad mitogenic and cell survival activities (Folkman & Klagsbrun; Kimelman & Kirschner) and are involved in a variety of biological processes, including cell proliferation, differentiation, survival, and apoptosis (Folkman & Klagsbrun; Klagsbrun; Rifkin & Moscatelli). FGF-8B signals through FGF receptors (FGFRs) to activate PI3K and MAPK pathways. FGF-8B regulates gastrulation, epithelial-mesenchymal transition, and mesenchymal to epithelial differentiation during embryonic development. FGF-8B has also been found in peripheral blood leukocytes and healthy bone marrow samples (Mattila & Härkönen). FGF-8B has mitogenic effects on somatic cells in the germinal epithelium and is expressed in adult mouse ovarian cells and tissues, which suggests that it regulates maturation of oocytes and seminiferous epithelium in testes (Valve et al.). This product is animal component-free.
Subtype
Cytokines, Growth Factors
Alternative Names
AIGF, Androgen-induced growth factor, FGF-8, Fibroblast growth factor-8B, HBGF-8, Heparin-binding growth factor-8
Cell Type
Endoderm, PSC-Derived, Hematopoietic Stem and Progenitor Cells, Neural Cells, PSC-Derived, Pluripotent Stem Cells
Species
Human
Area of Interest
Epithelial Cell Biology, Neuroscience, Stem Cell Biology
Molecular Weight
22.5 kDa
Purity
≥ 95%
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet 1 | Human Recombinant FGF-8B, ACF | 78204, 78204.1, 78204.2 | BX33850 or lower | English |
| Product Information Sheet 2 | Human Recombinant FGF-8B, ACF | 78204, 78204.1, 78204.2 | All other lots | English |
| Safety Data Sheet | Human Recombinant FGF-8B, ACF | 78204, 78204.1, 78204.2 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant FGF-8B, ACF was tested by its ability to promote the proliferation of NR6R 3T3 cells in the presence of heparin. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.915 ng/mL. (B) 1 μg of Human Recombinant FGF-8B, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant FGF-8B, ACF has a predicted molecular mass of 22.5 kDa.