Human Recombinant FGF-8B

Fibroblast growth factor 8B

概要技术资料数据及文献

概要

Fibroblast growth factor 8B (FGF-8B) is a member of the fibroblast growth factor (FGF) family and is an isoform of FGF-8. Cytokines in the FGF family possess broad mitogenic and cell survival activities (Folkman & Klagsbrun; Kimelman & Kirschner) and are involved in a variety of biological processes, including cell proliferation, differentiation, survival, and apoptosis (Folkman & Klagsbrun; Klagsbrun; Rifkin & Moscatelli). FGF-8B signals through FGF receptors (FGFRs) to activate PI3K and MAPK pathways. FGF-8B is broadly associated with mitogenic and cell survival activities, and regulates gastrulation, epithelial-mesenchymal transition, and later on mesenchymal to epithelial differentiation during embryonic development. FGF-8B has also been found in peripheral blood leukocytes and healthy bone marrow samples (Mattila & Härkönen). FGF-8B has mitogenic effects on somatic cells in the germinal epithelium and is expressed in adult mouse ovarian cells and tissues, which suggests that it regulates maturation of oocytes and seminiferous epithelium in testis (Valve et al.).

Cytokines, Growth Factors

AIGF, Androgen-induced growth factor, FGF-8, FGF-8B, Fibroblast growth factor-8B, HBGF-8, Heparin-binding growth factor 8; HH6, KAL6

Endoderm, PSC-Derived, Hematopoietic Stem and Progenitor Cells, Neural Cells, PSC-Derived, Pluripotent Stem Cells

Human

Epithelial Cell Biology, Neuroscience, Stem Cell Biology

22.5 kDa

> 95%

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Human Recombinant FGF-8B	78008, 78008.1, 78008.2	All	English
Safety Data Sheet	Human Recombinant FGF-8B	78008, 78008.1, 78008.2	All	English

数据及文献

Data

(A) The biological activity of Human Recombinant FGF-8B was tested by its ability to promote the proliferation of BALB/c 3T3 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 1.8 ng/mL.
(B) 2 μg of Human Recombinant FGF-8B was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant FGF-8B has a predicted molecular mass of 22.5 kDa.