Human Recombinant FGF-5, ACF

Fibroblast growth factor 5, animal component-free

概要技术资料数据及文献

概要

Fibroblast growth factor 5 (FGF-5) is a secreted, heparin-binding member of the FGF subfamily. FGFs possess broad mitogenic and cell survival activities and are expressed during embryonic development. FGF-5 is expressed in the mesenchyme, skeletal muscles, central nervous systems, and hair follicles. FGF-5 promotes cell differentiation and proliferation by binding FGF receptor 1 and FGF receptor 2 (FGFR1 and FGFR2, respectively). FGF-5 plays an important regulatory role in skeletal muscle development. FGF-5 has also been identified with neurons of the limbic system, especially those of the olfactory bulb and pyramidal cells of the hippocampus (Haub & Goldfarb). This product is animal component-free.

Cytokines, Growth Factors

Fibroblast growth factor 5, HBGF-5, Heparin-binding growth factor 5, Smag-82

Mesenchymal Stem and Progenitor Cells, Neurons

Human

Neuroscience, Stem Cell Biology

27.7 kDa

≥ 95%

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Human Recombinant FGF-5, ACF	78183, 78183.1, 78183.2	All	English
Safety Data Sheet	Human Recombinant FGF-5, ACF	78183, 78183.1, 78183.2	All	English

数据及文献

Data

(A) The biological activity of Human Recombinant FGF-5, ACF was tested by its ability to promote proliferation of NR6R-3T3 cells in the presence of 1 μg heparin. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 3.11 ng/mL. (B) 1 μg of Human Recombinant FGF-5, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant FGF-5, ACF has a predicted molecular mass of 27.7 kDa.