Human Recombinant Cardiotrophin-1

Cardiotrophin 1

概要技术资料数据及文献

概要

Cardiotrophin-1 is a member of the IL-6 (interleukin 6) cytokine family that includes IL-6, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). Cardiotrophin-1 is a pleiotropic cytokine with activity that overlaps with other IL-6 family members. Cardiotrophin-1 is expressed in various tissues including heart, skeletal muscle, liver, lung, kidney, ovary, colon, and prostate. It initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor β subunit (Pennica et al.; Robledo et al.). Binding of cardiotrophin-1 initiates signaling through the JAK/STAT, MEK/ERK, and AKT pathways. Cardiotrophin-1 has been shown to have cardioprotective and neuroprotective effects. It has also been shown to promote cardiomyocyte survival and plays an essential role in the development of neural tissue (López-Yoldi et al.).

Cytokines

CT-1, CTF1

Cardiomyocytes, PSC-Derived, Hepatic Cells, Prostate Cells

Human

Stem Cell Biology

21.1 kDa

≥ 95%

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Human Recombinant Cardiotrophin-1	78160, 78160.1	All	English
Safety Data Sheet	Human Recombinant Cardiotrophin-1	78160, 78160.1	All	English

数据及文献

Data

(A) The biological activity of Human Recombinant Cardiotrophin-1 was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is less than 0.4 ng/mL. (B) 2 μg of Human Recombinant Cardiotrophin-1 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant Cardiotrophin-1 has a predicted molecular mass of 21.1 kDa.