Human Recombinant Activin A, ACF
Activin A, animal component-free
概要
Activin A is a member of the transforming growth factor beta (TGF-β) family of proteins produced by many cell types throughout development (Gurdon et al.). It is a disulfide-linked homodimer (two beta-A chains) that binds to heteromeric complexes of a type I (Act RI-A and Act RI-B) and a type II (Act RII-A and Act RII-B) serine-threonine kinase receptor (Attisano et al.). Activins primarily signal through SMAD2/3 proteins to regulate a variety of functions, including cell proliferation, differentiation, wound healing, apoptosis, and metabolism (McDowell et al.). Activin A maintains the undifferentiated state of human embryonic stem cells (James et al.; Xiao et al.) and also facilitates differentiation of human embryonic stem cells into definitive endoderm (D’Amour et al.). This product is animal component-free.
Subtype
Cytokines
Alternative Names
Activin beta-A chain, EDF, Erythroid differentiation protein, FRP, FSH-releasing protein, INHBA, Inhibin betaA chain, Inhibin beta-1
Cell Type
Endoderm, PSC-Derived, Mesoderm, PSC-Derived, Other, Pluripotent Stem Cells
Area of Interest
Epithelial Cell Biology, Stem Cell Biology
Molecular Weight
13.1 kDa monomer; 26.2 kDa dimer
Purity
≥ 95%
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant Activin A, ACF | 78132, 78132.1, 78132.2 | All | English |
| Safety Data Sheet | Human Recombinant Activin A, ACF | 78132, 78132.1, 78132.2 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant Activin A, ACF was tested by its ability to inhibit proliferation of MPC-11 cells. Inhibition of cell proliferation was measured after 66 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation inhibition is at 50% of maximum. The EC50 in the example above is 0.436 ng/mL. (B) 1 μg of Human Recombinant Activin A, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.