Mouse Recombinant Shh

Sonic hedgehog

概要技术资料数据及文献

概要

Sonic hedgehog (Shh) is a member of the Hedgehog family of secreted signaling proteins that has a prominent role in patterning during early mammalian development (Ho & Scott). It is expressed during early embryogenesis in a variety of tissues (Finco et al.). During patterning of the central nervous system, Shh directly acts on cells to specify neural cell fates, regulates proliferation and survival of oligodendrocyte precursors and neural crest cells and stimulates proliferation, differentiation, and axon growth (Ho & Scott; Martí). Shh also regulates T cell differentiation and activation (Crompton et al.). Recombinant Mouse Shh is a fully biologically active molecule.

Cytokines

HHG-1, HLP3, HPE3, MCOPCB5, SMMCI, Sonic hedgehog, TPT, TPTPS

Neural Stem and Progenitor Cells, Pluripotent Stem Cells

Mouse

Neuroscience, Stem Cell Biology

19.8 kDa

≥ 95%

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Mouse Recombinant Shh	78066, 78066.1, 78066.2	All	English
Safety Data Sheet	Mouse Recombinant Shh	78066, 78066.1, 78066.2	All	English

数据及文献

Data

(A) The biological activity of Mouse Recombinant Shh was tested by its ability to promote alkaline phosphatase production of CCL-226 cells. Alkaline phosphatase production was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.8 μg/mL.
(B) 2 μg of Mouse Recombinant Shh was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant Shh has a predicted molecular mass of 19.8 kDa.