Costar® 12 mm Transwell®, 0.4 µm Pore Polyester Membrane Inserts

Polystyrene plate with lid and polyester membrane inserts
概要
Costar® 12 mm Transwell®, 0.4 µm Pore Polyester Membrane Inserts support the growth of both anchorage-dependent and anchorage-independent cells, including those that are fed at the basolateral surface or in co-culture systems. These permeable inserts are recommended for culturing airway epithelial cells at the air-liquid interface, and are available in a kit (Catalog #05021) that includes PneumaCult™-ALI Medium (Catalog #05001).

48 Inserts; 4 x 12-well plates, each with 12 inserts
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Cell Culture
技术资料
Document Type 产品名称 Catalog # Lot # 语言
Product Information Sheet Costar® 12 mm Transwell®, 0.4 µm Pore Polyester Membrane Inserts 38023 All English
数据及文献

Data

Figure 1. Representative Images of ALI Culture Morphology

A side-by-side comparison was performed between Transwell® Inserts and competitor inserts of the same material and pore size. HBECs were seeded onto the inserts at passage 3 (P3) and differentiated in PneumaCult™-ALI for 21 days. Air-liquid interface (ALI) cultures generated using PneumaCult™ and Transwell® Inserts provided the most optimal morphology.

Figure 2. Differential Epithelial Cell Marker Expression in ALI Cultures Generated with Different Inserts

HBECs were seeded onto the inserts at P3 and differentiated in PneumaCult™-ALI for 21 days. Gene expression of goblet (MUC5B) and ciliated (FOXJ1) cell markers was assessed by qPCR and normalized to beta actin. Comparison was made with competitor inserts of the same material and pore size. Higher expression of goblet and ciliated cell markers was observed when using PneumaCult™ together with Transwell® inserts.

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