Costar® 6.5 mm Transwell®, 0.4 µm Pore Polyester Membrane Inserts

Polystyrene plate with lid and polyester membrane inserts

概要技术资料数据及文献

概要

Costar® 6.5 mm Transwell®, 0.4 µm Pore Polyester Membrane Inserts are recommended for culturing airway epithelial cells at the air-liquid interface in PneumaCult™-ALI Medium (Catalog #05001). These permeable inserts support the growth of both anchorage-dependent and anchorage-independent cells, including those that are fed at the basolateral surface or in co-culture systems.

48 Inserts; 4 x 24-well plates, each with 12 inserts

Human, Mouse, Rat, Non-Human Primate, Other

Cell Culture

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Costar® 6.5 mm Transwell®, 0.4 µm Pore Polyester Membrane Inserts	38024	All	English

数据及文献

Data

Figure 1. Representative Images of ALI Culture Morphology

A side-by-side comparison was performed between Transwell® Inserts and competitor inserts of the same material and pore size. HBECs were seeded onto the inserts at passage 3 (P3) and differentiated in PneumaCult™-ALI for 21 days. Air-liquid interface (ALI) cultures generated using PneumaCult™ and Transwell® Inserts provided the most optimal morphology.

Figure 2. Differential Epithelial Cell Marker Expression in ALI Cultures Generated with Different Inserts

HBECs were seeded onto the inserts at P3 and differentiated in PneumaCult™-ALI for 21 days. Gene expression of goblet (MUC5B) and ciliated (FOXJ1) cell markers was assessed by qPCR and normalized to beta actin. Comparison was made with competitor inserts of the same material and pore size. Higher expression of goblet and ciliated cell markers was observed when using PneumaCult™ together with Transwell® inserts.