MesenCult™-ACF Freezing Medium
Animal component-free MSC cryopreservation medium
概要
MesenCult™-ACF Freezing Medium is a defined, serum-free, and animal component-free medium for the cryopreservation of mesenchymal stem cells (MSCs). This complete and ready-to-use medium is recommended for human MSCs previously cultured in MesenCult™-ACF Plus Medium (Catalog #05445) or MesenCult™ Medium (MesenCult™ Proliferation Kit; Catalog #05411). Frozen human MSCs should be stored at -135°C (liquid nitrogen) or colder.
Advantages
• Serum-free and animal component-free
• Reproducibly high recovery rates
• Optimized for MSCs previously cultured in MesenCult™-ACF Plus Medium or MesenCult™ Medium
• Preserves human MSC multipotency and expansion capacities
• Convenient, ready-to-use format
• Reproducibly high recovery rates
• Optimized for MSCs previously cultured in MesenCult™-ACF Plus Medium or MesenCult™ Medium
• Preserves human MSC multipotency and expansion capacities
• Convenient, ready-to-use format
Contains
Dimethyl sulfoxide (DMSO)
Cell Type
Mesenchymal Stem and Progenitor Cells
Species
Human
Application
Cryopreservation
Brand
MesenCult
Area of Interest
Stem Cell Biology
Formulation
Animal Component-Free, Serum-Free
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | MesenCult™-ACF Freezing Medium | 05490 | All | English |
| Safety Data Sheet | MesenCult™-ACF Freezing Medium | 05490 | All | English |
数据及文献
Data
Human MSCs previously cultured in animal component-free medium and cryopreserved in MesenCult™-ACF Freezing Medium show reproducibly high recovery of viable cells after thawing. Recovery is higher than observed with a competitor ACF freezing medium (n = 6 independent experiments, P = 0.0035). Viable cell recovery is defined as the percentage of input cells that are recovered and maintain post-thaw viability.
Human MSCs cryopreserved with MesenCult™-ACF Freezing Medium (A, 3 days after thawing) maintain multi-lineage differentiation potential, as demonstrated by differentiation to the adipogenic (B, Oil Red O staining) and osteogenic (C, Alizarin Red staining) lineages.