EasySep™ Mouse Biotin Positive Selection Kit II

Immunomagnetic positive selection kit
概要
The EasySep™ Mouse Biotin Positive Selection Kit II is designed to isolate cells that are labeled with biotinylated antibodies. Desired cells are targeted with antibody complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.

This product replaces the EasySep™ Mouse Biotin Positive Selection Kit (Catalog #18556).

For highly purified particle-free cells, we recommend the EasySep™ Release Mouse Biotin Positive Selection Kit (Catalog #17655).
Advantages
• Fast and easy-to-use
• No columns required
Components
  • EasySep™ Mouse Biotin Positive Selection Kit II (Catalog #17665)
    • EasySep™ Biotin Selection Cocktail, 1 mL
    • Mouse FcR Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
  • RoboSep™ Mouse Biotin Positive Selection Kit II (Catalog #17665RF)
    • EasySep™ Biotin Selection Cocktail, 1 mL
    • Mouse FcR Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Macrophages, Marrow Stromal Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Other, Plasma, T Cells
Species
Mouse
Sample Source
Bone Marrow, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
数据及文献

Data

Starting with mouse splenocytes, the purities of the start and final isolated fractions in the above example are 20.5% and 97.5%, respectively, using a biotinylated anti-mouse CD4 antibody and EasySep™ Mouse Biotin Positive Selection Kit II (as assessed using PE-conjugated streptavidin).

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