EasySep™ Mouse Biotin Positive Selection Kit II
Immunomagnetic positive selection kit
概要
The EasySep™ Mouse Biotin Positive Selection Kit II is designed to isolate cells that are labeled with biotinylated antibodies. Desired cells are targeted with antibody complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
This product replaces the EasySep™ Mouse Biotin Positive Selection Kit (Catalog #18556).
For highly purified particle-free cells, we recommend the EasySep™ Release Mouse Biotin Positive Selection Kit (Catalog #17655).
This product replaces the EasySep™ Mouse Biotin Positive Selection Kit (Catalog #18556).
For highly purified particle-free cells, we recommend the EasySep™ Release Mouse Biotin Positive Selection Kit (Catalog #17655).
Advantages
• Fast and easy-to-use
• No columns required
• No columns required
Components
- EasySep™ Mouse Biotin Positive Selection Kit II (Catalog #17665)
- EasySep™ Biotin Selection Cocktail, 1 mL
- Mouse FcR Blocker, 0.1 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
- RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
- RoboSep™ Mouse Biotin Positive Selection Kit II (Catalog #17665RF)
- EasySep™ Biotin Selection Cocktail, 1 mL
- Mouse FcR Blocker, 0.1 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
- RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Macrophages, Marrow Stromal Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Other, Plasma, T Cells
Species
Mouse
Sample Source
Bone Marrow, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
技术资料
Document Type | 产品名称 | Catalog # | Lot # | 语言 |
---|---|---|---|---|
Product Information Sheet | EasySep™ Mouse Biotin Positive Selection Kit II | 17665 | All | English |
Product Information Sheet | RoboSep™ Mouse Biotin Positive Selection Kit II | 17665RF | All | English |
Safety Data Sheet 1 | EasySep™ Mouse Biotin Positive Selection Kit II | 17665 | All | English |
Safety Data Sheet 2 | EasySep™ Mouse Biotin Positive Selection Kit II | 17665 | All | English |
Safety Data Sheet 3 | EasySep™ Mouse Biotin Positive Selection Kit II | 17665 | All | English |
Safety Data Sheet 1 | RoboSep™ Mouse Biotin Positive Selection Kit II | 17665RF | All | English |
Safety Data Sheet 2 | RoboSep™ Mouse Biotin Positive Selection Kit II | 17665RF | All | English |
Safety Data Sheet 3 | RoboSep™ Mouse Biotin Positive Selection Kit II | 17665RF | All | English |
数据及文献
Data
Starting with mouse splenocytes, the purities of the start and final isolated fractions in the above example are 20.5% and 97.5%, respectively, using a biotinylated anti-mouse CD4 antibody and EasySep™ Mouse Biotin Positive Selection Kit II (as assessed using PE-conjugated streptavidin).