EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit
概要
The EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit is designed to isolate CD138+ (syndecan-1) cells from whole blood and bone marrow by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD138 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD138 antigen is expressed on normal and malignant plasma cells (but not mature B cells).

This product replaces the EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit (Catalog #18387) for even faster cell isolations.
Advantages
• Fast and easy-to-use
• No columns required
Components
  • EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (Catalog #17887)
    • EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (Catalog #17887RF)
    • EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Myeloma, Plasma
Species
Human
Sample Source
Bone Marrow, Whole Blood
Selection Method
Positive
Application
ELISA
Brand
EasySep, RoboSep
Area of Interest
Immunology
数据及文献

Data

Starting with fresh whole blood spiked with a multiple myeloma cell line, U266, the CD138+ cell content of the selected fraction typically ranges from 83.7 - 98.3%. In the above example, the purities of the start and final isolated fractions are 9.1% and 90.4%, respectively. NOTE: For samples with CD138+ starting frequency < 10 - 15%, the CD138+ purity of the isolated fraction may be variable. NOTE: Red blood cells were removed from the start sample by lysis prior to flow cytometry.

Top