EasySep™ Human Whole Blood CD34 Positive Selection Kit II

Immunomagnetic positive selection kit
概要
The EasySep™ Human Whole Blood CD34 Positive Selection Kit II is designed to isolate CD34+ cells from fresh whole blood or freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD34 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells.

This product can be used instead of the EasySep™ Human Whole Blood CD34 Positive Selection Kit (Catalog #18086).

If isolating CD34+ cells from fresh cord blood, we recommend using the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (Catalog #17896). Alternatively, if isolating CD34+ cells from any other sample type, the EasySep™ Human CD34 Positive Selection Kit II (Catalog #17856) should be used.
Advantages
• Fast and easy-to-use
• No columns required
Components
  • EasySep™ Human Whole Blood CD34 Positive Selection Kit II (Catalog #17879)
    • EasySep™ Human Whole Blood CD34 Positive Selection Cocktail II, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ Human Whole Blood CD34 Positive Selection Kit II (Catalog #17879RF)
    • EasySep™ Human Whole Blood CD34 Positive Selection Cocktail II, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• "The Big Easy" EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Hematopoietic Stem and Progenitor Cells
Species
Human
Sample Source
Buffy Coat, Whole Blood
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, Immunology, Stem Cell Biology
数据及文献

Data

Starting with whole blood, the CD34+ cell content of the isolated fraction is typically 90.4 ± 7.0% (gated on CD45+ cells; mean ± SD using RoboSep™-S). In the above example, the purities of the start and final isolated fractions are 0.06% and 95.31%, respectively. NOTE: RBCs were removed by lysis prior to flow cytometry.

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