EasySep™ Human Plasmacytoid DC Isolation Kit
Immunomagnetic negative selection kit
概要
The EasySep™ Human Plasmacytoid DC Isolation Kit is designed to isolate plasmacytoid dendritic cells (pDCs) from human peripheral blood mononuclear cells (PBMCs) or leukapheresis by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
This product replaces the EasySep™ Human Plasmacytoid DC Enrichment Kit (Catalog #19062), for even faster cell isolations and is compatible with a greater number of EasySep™ magnets.
This product replaces the EasySep™ Human Plasmacytoid DC Enrichment Kit (Catalog #19062), for even faster cell isolations and is compatible with a greater number of EasySep™ magnets.
Advantages
⦁ Fast, easy-to-use, and column-free
⦁ Up to 97% purity
⦁ Untouched, viable cells
⦁ Up to 97% purity
⦁ Untouched, viable cells
Components
- EasySep™ Human Plasmacytoid DC Isolation Kit (Catalog #17977)
- EasySep™ Human Plasmacytoid DC Isolation Cocktail Component A, 2 x 1 mL
- EasySep™ Human Plasmacytoid DC Isolation Cocktail Component B, 2 x 1 mL
- EasySep™ Dextran RapidSpheres™ 50103, 4 x 1 mL
- Anti-Human CD32 (Fc gamma RII) Blocker, 2 x 0.8 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Dendritic Cells
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology, Infectious Diseases
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Human Plasmacytoid DC Isolation Kit | 17977 | All | English |
| Product Information Sheet | RoboSep™ Human Plasmacytoid DC Isolation Kit | 17977RF | All | English |
| Safety Data Sheet | EasySep™ Human Plasmacytoid DC Isolation Kit | 17977 | All | English |
| Safety Data Sheet | RoboSep™ Human Plasmacytoid DC Isolation Kit | 17977RF | All | English |
数据及文献
Data
Figure 1. Typical EasySep™ Human Plasmacytoid DC Isolation Profile
Starting with leukapheresis samples, the pDC content (Lin-HLA-DR+CD123+) of the isolated fraction is typically 90 ± 5.3% (mean ± SD using the silver “Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 0.2% and 89.8%, respectively.