EasySep™ Human Pan-Granulocyte Isolation Kit

Immunomagnetic negative selection cell isolation kit
概要
The EasySep™ Human Pan-Granulocyte Enrichment Kit is designed to isolate granulocytes (eosinophils, basophils and neutrophils) from normal peripheral whole blood by negative selection. Undesired cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-granulocytes and magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
Advantages
• Fast, easy-to-use and column-free
• Up to 99% purity
• Untouched, viable cells
Components
  • EasySep™ Human Pan-Granulocyte Isolation Kit (Catalog #19259)
    • EasySep™ Pan-Granulocyte Isolation Cocktail, 1 mL
    • EasySep™ Magnetic Particles, 2 x 1 mL
  • RoboSep™ Human Pan-Granulocyte Isolation Kit (Catalog #19259RF)
    • EasySep™ Pan-Granulocyte Isolation Cocktail, 1 mL
    • EasySep™ Magnetic Particles, 2 x 1 mL
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets
Species
Human
Sample Source
PMNC, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
数据及文献

Data

Typical EasySep™ Human Pan-Granulocyte Isolation Profile

Figure 1. Typical EasySep™ Human Pan-Granulocyte Isolation Profile

Starting with whole peripheral blood, the total granulocyte content of the isolated fraction typically ranges from 97 - 99%. As shown in the examples above, neutrophils are typically CD66b+CD16+. Eosinophils are typically CD66b+CD16- and basophils are CD66b- and can be further defined as CD203c+CD123+ and IgE+ (not shown).

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