EasySep™ Human Naïve Pan T Cell Isolation Kit

Immunomagnetic negative selection kit
概要
The EasySep™ Human Naïve Pan T Cell Isolation Kit is designed to isolate naïve pan T cells from fresh or previously frozen human peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Advantages
• Fast, easy-to-use and column-free
• Up to 98% purity
• Isolated cells are untouched
Components
    • EasySep™ Human Naïve Pan T Cell Isolation Kit (Catalog #17961)
        • EasySep™ Human Naïve Pan T Cell Isolation Cocktail, 1 mL
        • EasySep™ Human TCR Gamma/Delta Depletion Cocktail, 1 mL
        • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • RoboSep™ Human Naïve Pan T Cell Isolation Kit (Catalog #17961RF)
        • EasySep™ Human Naïve Pan T Cell Isolation Cocktail, 1 mL
        • EasySep™ Human TCR Gamma/Delta Depletion Cocktail, 1 mL
        • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
        • RoboSep™ Buffer (Catalog #20104)
        • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
数据及文献

Data

Starting with fresh PBMCs, the naïve pan T cell content (CD3+CD45RA+CD45RO-CD197+) of the isolated fraction is typically 96.1 ± 2.3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 4.5% and 97%, respectively.

Publications (1)

Science (New York, N.Y.) 2019 LMBR1L regulates lymphopoiesis through Wnt/beta-catenin signaling. J. H. Choi et al.
Abstract
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Abstract

Precise control of Wnt signaling is necessary for immune system development. In this study, we detected severely impaired development of all lymphoid lineages in mice, resulting from an N-ethyl-N-nitrosourea-induced mutation in the limb region 1-like gene (Lmbr1l), which encodes a membrane-spanning protein with no previously described function in immunity. The interaction of LMBR1L with glycoprotein 78 (GP78) and ubiquitin-associated domain-containing protein 2 (UBAC2) attenuated Wnt signaling in lymphocytes by preventing the maturation of FZD6 and LRP6 through ubiquitination within the endoplasmic reticulum and by stabilizing destruction complex" proteins. LMBR1L-deficient T cells exhibited hallmarks of Wnt/beta-catenin activation and underwent apoptotic cell death in response to proliferative stimuli. LMBR1L has an essential function during lymphopoiesis and lymphoid activation acting as a negative regulator of the Wnt/beta-catenin pathway."
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