EasySep™ Human Naïve CD8+ T Cell Isolation Kit II
Immunomagnetic negative selection kit
概要
The EasySep™ Human Naïve CD8+ T Cell Isolation Kit II is designed to isolate naïve CD8+ T cells from fresh peripheral blood mononuclear cells by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically-labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
This product replaces the EasySep™ Human Naïve CD8+ T Cell Enrichment Kit (Catalog #19158) for even faster cell isolations.
This product replaces the EasySep™ Human Naïve CD8+ T Cell Enrichment Kit (Catalog #19158) for even faster cell isolations.
Advantages
• Fast, easy-to-use and column-free
• Up to 97% purity
• Untouched, viable cells
• Up to 97% purity
• Untouched, viable cells
Components
- EasySep™ Human Naïve CD8+ T Cell Isolation Kit II (Catalog #17968)
- EasySep™ Human Naïve CD8+ T Cell Isolation Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™, 1 x 1 mL
- RoboSep™ Human Naive CD8+ T Cell Isolation Kit (Catalog #17968RF)
- EasySep™ Human Naïve CD8+ T Cell Isolation Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™, 1 x 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD8+
Species
Human
Sample Source
PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Human Naïve CD8+ T Cell Isolation Kit II | 17968 | All | English |
| Product Information Sheet | RoboSep™ Human Naïve CD8+ T Cell Isolation Kit II | 17968RF | All | English |
| Safety Data Sheet 1 | EasySep™ Human Naïve CD8+ T Cell Isolation Kit II | 17968 | All | English |
| Safety Data Sheet 2 | EasySep™ Human Naïve CD8+ T Cell Isolation Kit II | 17968 | All | English |
| Safety Data Sheet 3 | EasySep™ Human Naïve CD8+ T Cell Isolation Kit II | 17968 | All | English |
| Safety Data Sheet 1 | RoboSep™ Human Naïve CD8+ T Cell Isolation Kit II | 17968RF | All | English |
| Safety Data Sheet 2 | RoboSep™ Human Naïve CD8+ T Cell Isolation Kit II | 17968RF | All | English |
| Safety Data Sheet 3 | RoboSep™ Human Naïve CD8+ T Cell Isolation Kit II | 17968RF | All | English |
数据及文献
Data
Starting with fresh PBMCs, the naïve CD8+ T cell content (CD8+CD45RA+CCR7+ and CD45RO-CD57-CD56-) of the isolated fraction is typically 93.7 ± 2.4% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 6.8% and 94.2%, respectively.