EasySep™ Human Naïve B Cell Isolation Kit

9-Minute cell isolation kit using immunomagnetic negative selection

概要技术资料数据及文献

概要

The EasySep™ Human Naïve B Cell Isolation Kit is designed to isolate naïve B cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. This product can be used in place of the EasySep™ Human Naïve B Cell Enrichment Kit (Catalog #19254) for even faster cell isolations.

• Fast, easy-to-use and column-free
• Up to 98% purity with high recovery
• Isolated cells are untouched

EasySep™ Human Naïve B Cell Isolation Kit (Catalog #17254)

EasySep™ Human Naïve B Cell Isolation Cocktail, 1 mL
EasySep™ Dextran RapidSpheres™, 1 mL
EasySep™ Isolation Cocktail Enhancer, 1 mL

RoboSep™ Human Naïve B Cell Isolation Kit (Catalog #17254RF)

EasySep™ Human Naïve B Cell Isolation Cocktail, 1 mL
EasySep™ Dextran RapidSpheres™, 1 mL
EasySep™ Isolation Cocktail Enhancer, 1 mL
RoboSep™ Buffer (Catalog #20104)
RoboSep™ Filter Tips (Catalog #20125)

• EasySep™ Magnet (Catalog #18000)

• “The Big Easy” EasySep™ Magnet (Catalog #18001)

• Easy 50 EasySep™ Magnet (Catalog #18002)

• EasyEights™ EasySep™ Magnet (Catalog #18103)

• RoboSep™-S (Catalog #21000)

Cell Isolation Kits

B Cells

Human

Leukapheresis, PBMC

Negative

Cell Isolation

EasySep, RoboSep

Immunology

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	EasySep™ Human Naïve B Cell Isolation Kit	17254	Purchase date 2020-04-07 and later	English
Product Information Sheet 1	RoboSep™ Human Naïve B Cell Isolation Kit	17254RF	All other lots	English
Product Information Sheet 2	RoboSep™ Human Naïve B Cell Isolation Kit	17254RF	Purchase date 2020-04-07 and later	English
Safety Data Sheet 1	EasySep™ Human Naïve B Cell Isolation Kit	17254	All	English
Safety Data Sheet 2	EasySep™ Human Naïve B Cell Isolation Kit	17254	All	English
Safety Data Sheet 3	EasySep™ Human Naïve B Cell Isolation Kit	17254	All	English
Safety Data Sheet 4	EasySep™ Human Naïve B Cell Isolation Kit	17254	All	English
Safety Data Sheet 1	RoboSep™ Human Naïve B Cell Isolation Kit	17254RF	All	English
Safety Data Sheet 2	RoboSep™ Human Naïve B Cell Isolation Kit	17254RF	All	English
Safety Data Sheet 3	RoboSep™ Human Naïve B Cell Isolation Kit	17254RF	All	English

数据及文献

Data

Starting with human PBMCs, the naïve B cell (CD3-CD19+CD27-) content of the isolated fraction is typically 94.9 ± 2.2% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 7.1% and 97.7%, respectively.