EasySep™ Human Myeloid Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit
概要
The EasySep™ Human Myeloid Positive Selection Kit II is designed to isolate CD33+ and CD66b+ cells from ammonium chloride lysed blood or bone marrow by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD33, CD66b and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD33 and CD66b antigens are expressed on myeloid progenitors monocytes and granulocytes.


This product replaces the EasySep™ Human Myeloid Positive Selection Kit (Catalog #18653) for even faster cell isolations.
Advantages
• Fast and easy-to-use
• Up to 99% purity
• No columns required
Components
  • EasySep™ Human Myeloid Positive Selection Kit II (Catalog #17893)
    • EasySep™ Human Myeloid Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
  • RoboSep™ Human Myeloid Positive Selection Kit II with Filter Tips (Catalog #17893RF)
    • EasySep™ Human Myeloid Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets, Myeloid Cells
Species
Human
Sample Source
PMNC, Whole Blood
Selection Method
Positive
Brand
EasySep, RoboSep
Area of Interest
Chimerism, HLA, Immunology
数据及文献

Data

Starting with peripheral blood MNCs, the myeloid cell content (CD14+CD66b+) of the isolated fraction is typically 96.5 ± 1.3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 59.2% and 94.6%, respectively.

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