EasySep™ Human Memory CD8+ T Cell Enrichment Kit

Immunomagnetic negative selection kit
概要
The EasySep™ Human Memory CD8+ T Cell Enrichment Kit is designed to isolate memory CD8+ T cells from peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with antibody complexes recognizing CD4, CD14, CD16, CD19, CD20, CD34, CD36, CD45RA, CD56, CD61, CD66b, CD123, TCRγ/δ, glycophorin A and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
Advantages
• Fast, easy-to-use and column-free
• Up to 92% purity
• Untouched, viable cells
Components
  • EasySep™ Human Memory CD8+ T Cell Enrichment Kit (Catalog #19159)
    • EasySep™ Memory CD8+ T Cell Enrichment Cocktail, 1 mL
    • EasySep™ Magnetic Particles, 3 x 1 mL
  • RoboSep™ Human Memory CD8+ T Cell Enrichment Kit (Catalog #19159RF)
    • EasySep™ Memory CD8+ T Cell Enrichment Cocktail, 1 mL
    • EasySep™ Magnetic Particles, 3 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD8+
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
数据及文献

Data

Typical EasySep™ Human Memory CD8+ T Cell Enrichment Profile

Figure 1. Typical EasySep™ Human Memory CD8+ T Cell Enrichment Profile

Starting with mononuclear cells, the memory CD8+ T cell (CD8+CD45RA-CD45RO+) content of the enriched fraction typically ranges from 72 - 92%. In the example above, the purities of the start and enriched fractions are 4.9% and 88.8%, respectively.

Publications (1)

Nature communications 2018 OCT Human breast tumor-infiltrating CD8+ T cells retain polyfunctionality despite PD-1 expression. C. A. Egelston et al.
Abstract
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Abstract

Functional CD8+ T cells in human tumors play a clear role in clinical prognosis and response to immunotherapeutic interventions. PD-1 expression in T cells involved in chronic infections and tumors such as melanoma often correlates with a state of T-cell exhaustion. Here we interrogate CD8+ tumor-infiltrating lymphocytes (TILs) from human breast and melanoma tumors to explore their functional state. Despite expression of exhaustion hallmarks, such as PD-1 expression, human breast tumor CD8+ TILs retain robust capacity for production of effector cytokines and degranulation capacity. In contrast, melanoma CD8+ TILs display dramatic reduction of cytokine production and degranulation capacity. We show that CD8+ TILs from human breast tumors can potently kill cancer cells via bi-specific antibodies. Our data demonstrate that CD8+ TILs in human breast tumors retain polyfunctionality, despite PD-1 expression, and suggest that they may be harnessed for effective immunotherapies.
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