EasySep™ Human IgG+ Memory B Cell Isolation Kit

Immunomagnetic cell isolation kit using particle release technology
概要
The EasySep™ Human IgG+ Memory B Cell Isolation Kit isolates IgG+CD27+ B cells from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or leukapheresis samples. First, CD27+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD27+ cells, and unwanted non-B cells, as well as IgM+, IgD+ and IgA+ cells are depleted using EasySep™ Dextran RapidSpheres™. The final isolated fraction contains IgG+CD27+ memory B cells, which readily secrete IgG in culture supernatants in the presence of appropriate stimuli.
Advantages
• Highly purified IgG+ memory B cells isolated in less than 40 minutes
• No-wash removal of EasySep™ Releasable RapidSpheres™
• Optional isolation of untouched naïve B cells from the same sample
Components
  • EasySep™ Human IgG+ Memory B Cell Isolation Kit (Catalog #17868)
    • EasySep™ Human CD27 Positive Selection Cocktail, 1 mL
    • EasySep™ Releasable RapidSpheres™, 2 x 1 mL
    • EasySep™ Release Buffer, 2 x 1 mL
    • EasySep™ Human Memory B Cell Pre-Enrichment Cocktail, 2 x 1 mL
    • EasySep™ Human IgM/IgD/IgA Depletion Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology
数据及文献

Data

FACS Profile Results with EasySep™ Human IgG+ Memory B Cell Isolation Kit

Figure 1. EasySep™ Human IgG+ Memory B Cell Isolation Kit

Starting with PBMCs, the IgG+ memory B cell content (CD19+CD27+IgG+) of the isolated fraction is typically 82.6 ± 7.0% (mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and isolated fractions are 1.0% and 90.2% respectively.

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