EasySep™ Human ILC2 Isolation Kit
Immunomagnetic positive selection kit using particle release technology
概要
The EasySep™ Human ILC2 Isolation Kit is designed to isolate Group 2 Innate Lymphoid Cells (ILC2s) from leukapheresis products by immunomagnetic positive selection without the use of a flow sorter.
First, CRTH2+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CRTH2+ cells, and unwanted non-ILC2s are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified ILC2s that are immediately ready for downstream applications.
Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
First, CRTH2+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CRTH2+ cells, and unwanted non-ILC2s are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified ILC2s that are immediately ready for downstream applications.
Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Advantages
• Easy-to-use and column-free
• Up to 95% purity
• Facilitates rapid isolation of ILC2s without flow sorting
• No-wash removal of EasySep™ Releasable RapidSpheres™
• Up to 95% purity
• Facilitates rapid isolation of ILC2s without flow sorting
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components
- EasySep™ Human ILC2 Isolation Kit (Catalog #17782)
- EasySep™ Human CRTH2-PE Positive Selection Cocktail, 0.1 mL
- EasySep™ Release PE Positive Selection Cocktail, 2 x 0.5mL
- EasySep™ Releasable RapidSpheres™ 50201, 1 mL
- EasySep™ Release Buffer, 1 mL
- EasySep™ Human ILC2 Isolation Cocktail, 0.5 mL
- EasySep™ Dextran RapidSpheres™ 50103, 1 mL
- Anti-Human CD32 Blocker, 1 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
Subtype
Cell Isolation Kits
Cell Type
Innate Lymphoid Cells
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology
技术资料
Document Type | 产品名称 | Catalog # | Lot # | 语言 |
---|---|---|---|---|
Product Information Sheet | EasySep™ Human ILC2 Isolation Kit | 17782 | All | English |
Safety Data Sheet 1 | EasySep™ Human ILC2 Isolation Kit | 17782 | All | English |
Safety Data Sheet 2 | EasySep™ Human ILC2 Isolation Kit | 17782 | All | English |
Safety Data Sheet 3 | EasySep™ Human ILC2 Isolation Kit | 17782 | All | English |
Safety Data Sheet 4 | EasySep™ Human ILC2 Isolation Kit | 17782 | All | English |
Safety Data Sheet 5 | EasySep™ Human ILC2 Isolation Kit | 17782 | All | English |
Safety Data Sheet 6 | EasySep™ Human ILC2 Isolation Kit | 17782 | All | English |
数据及文献
Data
Figure 1. Typical EasySep™ Human ILC2 Isolation Profile
Starting with fresh leukapheresis samples, the ILC2 content (CD45+Lin- CRTH2+CD161+CD127+) of the isolated fraction typically ranges from 84 - 95%. In the above example, the purities of the start and final isolated fractions are 0.03% and 91% (gated on CD45), respectively.