EasySep™ Human ILC2 Enrichment Kit

Immunomagnetic negative selection cell enrichment kit
概要
The EasySep™ Human ILC2 enrichment kit is designed to enrich Group 2 Innate Lymphoid Cells (ILC2s) from leukapheresis products by immunomagnetic negative selection. Unwanted cells are targeted for removal by Tetrameric Antibody Complexes directed against non-ILC2s and dextran-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube as desired cells are simply poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry and cell sorting.
Advantages
• Fast, easy-to-use and column-free
• Untouched, viable cells
• Facilitates rapid flow sorting of ILC2s
Components
  • EasySep™ Human ILC2 Enrichment Kit (Catalog #17972)
    • EasySep™ Human ILC2 Enrichment Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50103, 1 mL
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
 
Subtype
Cell Isolation Kits
Cell Type
Innate Lymphoid Cells
Species
Human
Sample Source
Leukapheresis
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology
数据及文献

Data


Figure 1. Typical EasySep™ Human ILC2 Enrichment Profile

Starting with washed leukapheresis sample, the ILC2 content (Lin-CD45+CD294+CD127+CD161+) of the enriched fraction typically ranges from 13 - 78%. In the above example, the percentages of ILC2s in the start and final enriched fractions are 0.03% and 35.3%, respectively. NOTE: The ILC2 content of the start fraction typically ranges from 0.001 - 0.16%.

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