EasySep™ Human Cord Blood CD34 Positive Selection Kit III

Immunomagnetic positive selection kit
概要
Isolate highly purified CD34+ cells from fresh whole umbilical cord blood (CB) using a simple, two-step procedure. First, hematopoietic progenitor cells are pre-enriched using the RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail (15226C) with antibodies recognizing T cell, B cell and myeloid cell surface markers. CD34+ cells are then selected using the EasySep™ Human CD34 Positive Selection Cocktail (18096C), which contains an antibody recognizing CD34.

This kit does not deplete platelets. If isolating CD34+ cells from whole CB in which platelet contamination is expected the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (Catalog #17896) is recommended.

If isolating CD34+ cells from fresh blood or buffy coat, the Complete Kit for Human Whole Blood CD34+ Cells (Catalog #15086) is recommended.

If isolating CD34+ cells from other samples, including fresh or previously frozen mobilized peripheral blood or bone marrow mononuclear cells, or from previously frozen cord blood mononuclear cells, we recommend using the EasySep™ Human CD34 Positive Selection Kit II (Catalog #17856).
Advantages
• Fast and easy-to-use
• Up to 96% purity
• No columns required
• Can be combined with SepMate™ for consistent, high-throughput sample processing
Components
  • EasySep™ Human Cord Blood CD34 Positive Selection Kit III (Catalog #17897)
    • RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail, 2 x 2.5 mL
    • EasySep™ Human CD34 Positive Selection Cocktail, 2 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
  • RoboSep™ Human Cord Blood CD34 Positive Selection Kit III (Catalog #17897RF)
    • RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail, 2 x 2.5 mL
    • EasySep™ Human CD34 Positive Selection Cocktail, 2 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™ (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Hematopoietic Stem and Progenitor Cells
Species
Human
Sample Source
Cord Blood
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep, RosetteSep
Area of Interest
Immunology, Stem Cell Biology
数据及文献

Data

Figure 1. Typical EasySep™ Human Cord Blood CD34 Positive Selection III Isolation Profile

Starting with fresh cord blood, the CD34+ cell content of the isolated fraction is typically 87 ± 12% (mean ± SD, n=10, data obtained using the purple EasySep™ Magnet). The CD34+ cell content of the starting sample is typically 0.5 ± 0.25% (mean ± SD).

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