EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit
Immunomagnetic cell isolation kit using particle release technology
概要
This kit isolates human CD62L+CD45RO+ and CD62L-CD45RO+ memory CD4+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. First, CD62L+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD62L+ cells, and unwanted CD45RA+ and non-CD4+ T cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified CD62L+CD45RO+ central memory CD4+ T cells that are immediately ready for downstream applications. Effector memory CD4+ T cells can be isolated from the CD62L- fraction.
Advantages
• Easy-to-use and column-free
• Up to 96% purity
• No-wash removal of EasySep™ Releasable RapidSpheres™
• Up to 96% purity
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components
- EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit (Catalog #17865)
- EasySep™ Human Memory CD4+ T Cell Isolation Cocktail, 0.5 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
- EasySep™ Human CD62L Positive Selection Cocktail, 1 mL
- EasySep Human CD25 Depletion Cocktail, 1 mL
- EasySep™ Releasable RapidSpheres™, 1 mL
- EasyeSep™ Release Buffer, 1 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
T Cells, CD4+
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit | 17865 | All | English |
| Safety Data Sheet 1 | EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit | 17865 | All | English |
| Safety Data Sheet 2 | EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit | 17865 | All | English |
| Safety Data Sheet 3 | EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit | 17865 | All | English |
| Safety Data Sheet 4 | EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit | 17865 | All | English |
| Safety Data Sheet 5 | EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit | 17865 | All | English |
| Safety Data Sheet 6 | EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit | 17865 | All | English |
数据及文献
Data
Starting with fresh PBMCs, the central memory CD4+ T cell content (CD3+CD4+CD45RO+CD62L+) of the isolated fraction is typically 92.3 ± 3.9% (mean ± SD using the purple EasySep™ magnet). The effector memory content (CD3+CD4+CD45RO+CD62L-) of the isolated fraction is typically 92.4 ± 4.1% (mean ± SD).
NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.