EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit

Immunomagnetic negative selection kit
概要
The EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit is designed to isolate CD4+CD127lowCD49d- cells from fresh peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-CD4+ T cells, CD127high, CD49d+ cells and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. The desired cells are poured off into a new tube.
Advantages
• Fast, easy-to-use and column-free
• Up to 87.4% purity
• Isolated cells are untouched
Components
  • EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit (Catalog #19232)
    • EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Cocktail, 1 mL
    • EasySep™ Dextran Selection Cocktail, 1 mL
    • EasySep™ Magnetic Particles, 3 x 1 mL
  • RoboSep™ CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit (Catalog #19232RF)
    • EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Cocktail, 1 mL
    • EasySep™ Dextran Selection Cocktail, 1 mL
    • EasySep™ Magnetic Particles, 3 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD4+, T Cells, Regulatory
Species
Human
Sample Source
PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
数据及文献

Data

Typical EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Profile

Figure 1. Typical EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Profile

Starting with fresh PBMCs, the CD3+CD4+CD127lowCD49d-CD25+FOXP3+ cell content of the enriched fraction typically ranges from 57.4 - 87.4%. Donor to donor variability is unavoidable and may affect the purity of the enriched fraction. In the example above, the purities of the start and enriched fractions are 1.84% and 84.1%, respectively.

Publications (1)

The Journal of Immunology 2015 A Transendocytosis Model of CTLA-4 Function Predicts Its Suppressive Behavior on Regulatory T Cells Hou TZ et al.
Abstract
View publication

Abstract

Manipulation of the CD28/CTLA-4 pathway is at the heart of a number of immunomodulatory approaches used in both autoimmunity and cancer. Although it is clear that CTLA-4 is a critical regulator of T cell responses, the immunological contexts in which CTLA-4 controls immune responses are not well defined. In this study, we show that whereas CD80/CD86-dependent activation of resting human T cells caused extensive T cell proliferation and robust CTLA-4 expression, in this context CTLA-4 blocking Abs had no impact on the response. In contrast, in settings where CTLA-4(+) cells were present as regulators�
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