EasySep Human CD2 Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit
概要
The EasySep™ Human CD2 Positive Selection Kit II is designed to isolate CD2+ cells from fresh or previously frozen human peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD2 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD2 antigen is expressed on T cells and NK cells.

This product replaces the EasySep™ Human CD2 Positive Selection Kit (Catalog #18657) for even faster cell isolations.
Advantages
• Fast and easy-to-use
• Up to 97% purity
• No columns required
Components
  • EasySep™ Human CD2 Positive Selection Kit (Catalog #17883)
    • EasySep™ Human CD2 Positive Selection Cocktail II, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 1 mL
  • RoboSep™ Human CD2 Positive Selection Kit (Catalog #17883RF)
    • EasySep™ Human CD2 Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Subtype
Cell Isolation Kits
Cell Type
T Cells
Species
Human
Sample Source
PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, HLA, Immunology
数据及文献

Data

Starting with PBMCs, the CD2+ cell content of the isolated fraction is typically 93.8 ± 3.3% (mean ± SD using the purple EasySep™ Magnet; as assessed by labeling with CD3 and CD56). In the above example, the purities of the start and final isolated fractions are 66.1% and 94.8%, respectively.

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