EasySep™ Non-Human Primate CD8+ T Cell Isolation Kit

25-Minute cell isolation kit using immunomagnetic negative selection
概要
The EasySep™ Non-Human Primate CD8+ T Cell Isolation Kit is designed to isolate CD8+ T cells from fresh peripheral blood mononuclear cells by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.

This kit has been verified for use with rhesus and cynomolgus macaques.
Advantages
⦁ Fast, easy to use, and column-free
⦁ Up to 85% purity with high recovery
⦁ Untouched, viable cells
Components
  • EasySep™ Non-Human Primate CD8+ T Cell Isolation Kit (Catalog #19583)
    • EasySep™ Non-Human Primate CD8+ T Cell Isolation Cocktail, 1.2 mL
    • EasySep™ D Magnetic Particles, 3 x 1 mL
  • RoboSep™ Non-Human Primate CD8+ T Cell Isolation Kit (Catalog #19583RF)
    • EasySep™ Non-Human Primate CD8+ T Cell Isolation Cocktail, 1.2 mL
    • EasySep™ D Magnetic Particles, 3 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Cell Type
T Cells, CD8+
Species
Non-Human Primate
Sample Source
PBMC, Peripheral Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
HIV, Immunology, Infectious Diseases
数据及文献

Data

The content of rhesus macaque CD8+ T cells before and after isolation correspond to 55.7% and 93.9%, respectively.

Figure 1. EasySep™ Non-Human Primate CD8+ T Cell Isolation Kit

In this example, starting with rhesus macaque PBMCs, the CD8+ T cell content (within the CD45+CD3+ gate) of start and final isolated fractions are 50.7% and 94.1%, respectively.

Top