EasySep™ Human Biotin Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit
概要
The EasySep™ Human Biotin Positive Selection Kit II is designed to isolate cells that are labeled with biotinylated antibodies. Desired cells are targeted with antibody complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.

This product replaces the EasySep™ Human Biotin Positive Selection Kit (Catalog #18553).

For highly purified particle-free cells, we recommend the EasySep™ Release Human Biotin Positive Selection Kit (Catalog #17653).
Advantages
• Fast and easy-to-use
• No columns required
Components
  • EasySep™ Human Biotin Positive Selection Kit II (Catalog #17663)
    • EasySep™ Biotin Selection Cocktail, 1 mL
    • Anti-Human CD32 FcR Blocker, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
  • RoboSep™ Human Biotin Positive Selection Kit II (Catalog #17663RF)
    • EasySep™ Biotin Selection Cocktail, 1 mL
    • Anti-Human CD32 FcR Blocker, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Macrophages, Marrow Stromal Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Other, Plasma, T Cells
Species
Human
Sample Source
Buffy Coat, Cord Blood, Leukapheresis, Other, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
数据及文献

Data

Starting with human PBMCs, the purities of the start and final isolated fractions in the above example are 60% and 97.4%, respectively, using a biotinylated anti-human CD3 antibody and EasySep™ Human Biotin Positive Selection Kit II (as assessed using a PE-conjugated anti-human CD3 antibody).

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