EasySep™ Human Basophil Isolation Kit

13-Minute cell isolation kit using immunomagnetic negative selection
概要
The EasySep™ Human Basophil Isolation Kit is designed to isolate basophils from a polymorphonuclear cell-rich fraction by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-basophils and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.

The isolation kit is compatible with cells prepared using HetaSep™ (Catalog #07906) sedimentation of fresh peripheral blood.

This product replaces the EasySep™ Human Basophil Enrichment Kit (Catalog #19069) for even faster cell isolations.
Advantages
• Fast, easy-to-use and column-free
• Up to 98% purity
• Isolated cells are untouched
Components
  • EasySep™ Human Basophil Isolation Kit (Catalog #17969)
    • EasySep™ Human Basophil Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
  • RoboSep™ Human Basophil Isolation Kit with Filter Tips (Catalog #17969RF)
    • EasySep™ Human Basophil Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets
Species
Human
Sample Source
PMNC, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
数据及文献

Data

Figure 1. Typical EasySep™ Human Basophil Isolation Profile

Starting with HetaSep™-processsed human peripheral blood, the basophil content (CD45+CD123+IgE+) of the isolated fraction is typically 94.0 ± 2.5% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 0.86% and 95.6% (gated on CD45+), respectively.

Top