EasySep™ HLA Total Lymphocyte Enrichment: Complete Processing Kit for Whole Blood

Immunomagnetic negative selection cell isolation kit
概要
The EasySep™ Total Lymphocyte Enrichment Kit: Complete Processing Kit for Whole Blood is designed to isolate human lymphocytes from HetaSep™-treated whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD14, CD15, CD16, CD33, CD36, CD41, CD66b, glycophorin A and dextran-coated magnetic particles. The labeled cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
Advantages
• Fast, easy-to-use and column-free
• Up to 97% purity
• Untouched, viable cells
• Compatible with downstream HLA assays
Components
  • EasySep™ HLA Total Lymphocyte Enrichment: Complete Processing Kit for Whole Blood (Catalog #19961HLA)
    • EasySep™ HLA WB Total Lymphocyte Enrichment Cocktail, 1 mL
    • EasySep™ D Magnetic Particles, 3 x 1 mL
    • HetaSep™, 20 mL (Catalog #07806) x 2
  • RoboSep™ HLA Total Lymphocyte Enrichment: Complete Processing Kit for Whole Blood with Filter Tips (Catalog #19961HLARF)
    • EasySep™ HLA WB Total Lymphocyte Enrichment Cocktail, 1 mL
    • EasySep™ D Magnetic Particles, 3 x 1 mL
    • HetaSep™, 20 mL (Catalog #07806) x 2
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Lymphocytes
Species
Human
Sample Source
Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, HLA, Immunology
数据及文献

Data

Typical EasySep™ Total Lymphocyte Enrichment Profile

Figure 1. Typical EasySep™ Total Lymphocyte Enrichment Profile

Starting with HetaSep™-treated whole blood, the CD3+ / CD19+ cell content of the enriched fraction typically ranges from 90.2 - 96.9% (gated on CD45+ cells). Starting with 0.5 mL of HetaSep™-treated whole blood (equivalent to 5 mL of whole blood), 1.3 - 3.6 x 10e6 CD3+ or CD19+ cells are typically recovered.

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