EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit
概要
The EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit is designed to isolate highly purified myeloid (including CD14+ and CD66b+) cells from fresh whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD33 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD33 antigen is expressed on myeloid progenitor cells, granulocytes and monocytes.

This product replaces the EasySep™ Human Whole Blood CD33 Positive Selection Kit (Catalog #18287) and EasySep™ HLA Whole Blood CD33 Positive Selection Kit (Catalog #18287HLA) for even faster cell isolations.
Advantages
• Fast and easy-to-use
• Up to 99% purity
• No columns required
Components
  • EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit (Catalog #17885)
    • EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit with Filter Tips (Catalog #17885RF)
    • EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets, Myeloid Cells
Species
Human
Sample Source
Buffy Coat, Whole Blood
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Chimerism, HLA, Immunology
数据及文献

Data

Starting with fresh whole blood, the CD33+ cell content of the isolated fraction is typically 96 ± 2% (mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start (as assessed by labeling with anti-CD33) and the final isolated fractions are 68.0% and 96.2% (as assessed by labeling with anti-CD14 and anti-CD66b), respectively.
NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.

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