EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit
概要
The EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection is designed to isolate CD4+ cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD4 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD4 antigen is expressed on helper T cells and more weakly on monocytes and tissue macrophages.

This product replaces the EasySep™ Human Whole Blood CD4 Positive Selection Kit (Catalog #18082) for even faster cell isolations.
Advantages
• Fast and easy-to-use
• Up to 99% purity
• No columns required
Components
  • EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit (Catalog #17888)
    • EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ (Catalog #50100), 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit with Filter Tips (Catalog #17888RF)
    • EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ (Catalog #50100), 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep Magnet™ (Catalog #18103)
• RoboSep™-S (Catalog #21000)
 
Subtype
Cell Isolation Kits
Cell Type
T Cells, CD4+
Species
Human
Sample Source
Buffy Coat, Peripheral Blood
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, HLA, Immunology
数据及文献

Data

Starting with human whole blood, the CD4+CD14- cell content of the isolated fraction is typically 97.0 ± 1.8% (gated on CD45; mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 29.8% and 99.0%, respectively. NOTE: RBCs were removed from the start sample by ammonium chloride lysis prior to flow cytometry.

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