EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit
概要
The EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit is designed to isolate CD14+ cells from freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD14 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD14 antigen is strongly expressed on monocytes and is weakly expressed on granulocytes. It is also expressed on most tissue macrophages.

This product replaces the EasySep™ Human Buffy Coat CD14 Positive Selection Kit (Catalog #18088) for even faster cell isolations.
Advantages
• Fast and easy-to-use
• Up to 99% purity
• No columns required
Components
  • EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit (Catalog #17878)
    • EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Cocktail, 2 x 0.75 mL
    • EasySep™ Dextran RapidSpheres 50100, 2 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit with Filter Tips (Catalog #17878RF)
    • EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Cocktail, 2 x 0.75 mL
    • EasySep™ Dextran RapidSpheres, 50100, 2 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
 
Subtype
Cell Isolation Kits
Cell Type
Monocytes
Species
Human
Sample Source
Buffy Coat
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, Immunology
数据及文献

Data

Starting with buffy coat, the CD14+ cell content of the isolated fraction is typically 93.0 ± 6.4% (mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 8.9% and 98.4%, respectively.

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