EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood
Immunomagnetic negative selection cell isolation kit
概要
The EasySep™ HLA B Cell Enrichment Kit: Complete Processing Kit for Whole Blood is designed to isolate human B cells from HetaSep™-treated whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD14, CD16, CD33, CD36, CD41, CD43, CD56, CD66b, glycophorin A and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
Advantages
• Fast, easy-to-use and column-free
• Up to 99% purity
• Untouched, viable cells
• Compatible with downstream HLA assays
• Up to 99% purity
• Untouched, viable cells
• Compatible with downstream HLA assays
Components
- EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood (Catalog #19954HLA)
- EasySep™ HLA B Cell Enrichment Cocktail, 1 mL
- EasySep™ Magnetic Particles, 4 x 1 mL
- HetaSep™, 20 mL (Catalog #07806) x 2
- RoboSep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood with Filter Tips (Catalog #19954HLARF)
- EasySep™ HLA B Cell Enrichment Cocktail, 1 mL
- EasySep™ Magnetic Particles, 4 x 1 mL
- HetaSep™, 20 mL (Catalog #07806) x 2
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Human
Sample Source
Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, HLA, Immunology
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood | 19954HLA | All | English |
| Product Information Sheet | RoboSep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood with Filter Tips | 19954HLARF | All | English |
| Safety Data Sheet 1 | EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood | 19954HLA | All | English |
| Safety Data Sheet 2 | EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood | 19954HLA | All | English |
| Safety Data Sheet 3 | EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood | 19954HLA | All | English |
| Safety Data Sheet 1 | RoboSep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood with Filter Tips | 19954HLARF | All | English |
| Safety Data Sheet 2 | RoboSep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood with Filter Tips | 19954HLARF | All | English |
数据及文献
Data
Figure 1. FACS Results with EasySep™ HLA B Cell Enrichment Kit
Starting with HetaSep™-treated whole blood, the CD19+ cell content of the enriched fraction typically ranges from 81.5 - 99.7% (gated on CD45+ cells). Starting with 0.5 mL of HetaSep™-treated whole blood (equivalent to 5 mL of whole blood), 1.2 - 9.1 x 10e5 CD19+ cells are typically recovered.