EasySep™ Direct Human T Cell Isolation Kit

Immunomagnetic negative selection from whole blood kit

概要技术资料数据及文献

概要

This cell separation kit can be used to isolate highly purified T cells directly from human whole blood by immunomagnetic negative selection.

This kit targets non-T cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and can directly be used for functional assays, flow cytometry or DNA/RNA extraction.

• > 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation, or lysis
• Up to 97% purity of isolated cells
• Fast, easy-to-use and column-free
• Isolated cells are untouched

EasySep™ Direct Human T Cell Isolation Kit (Catalog #19661)

EasySep™ Direct Human T Cell Isolation Cocktail, 2 x 2.5 mL
EasySep™ Direct RapidSpheres™, 4 x 2.5 mL

RoboSep™ Human T Cell Isolation Kit with Filter Tips (Catalog #19661RF)

EasySep™ Direct Human T Cell Isolation Cocktail, 2 x 2.5 mL
EasySep™ Direct RapidSpheres™, 4 x 2.5 mL

RoboSep™ Buffer (Catalog #20104)

RoboSep™ Filter Tips (Catalog #20125) x 2

• EasySep™ Magnet (Catalog #18000)

• “The Big Easy” EasySep™ Magnet (Catalog #18001)

• Easy 50 EasySep™ Magnet (Catalog #18002)

• EasyEights™ EasySep™ Magnet (Catalog #18103)

• RoboSep™-S (Catalog #21000)

Cell Isolation Kits

T Cells

Human

Whole Blood

Negative

Cell Isolation

EasySep, RoboSep

Cell Therapy, Immunology

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	EasySep™ Direct Human T Cell Isolation Kit	19661	18F92420 or higher	English
Product Information Sheet	EasySep™ Direct Human T Cell Isolation Kit for RoboSep™	19661RF	18F92420 or higher	English
Safety Data Sheet 1	EasySep™ Direct Human T Cell Isolation Kit	19661	All	English
Safety Data Sheet 2	EasySep™ Direct Human T Cell Isolation Kit	19661	All	English

数据及文献

Data

Isolated T cells from whole blood obtained using EasySep™ Direct human T cell isolation kit

Figure 1. Typical EasySep™ Direct Human T Cell Isolation Profile

Starting with human whole blood from normal healthy donors, the typical T cell (CD3+) content of the non-lysed final isolated fraction is 95.3 ± 1.4% (gated on CD45) or 94.9 ± 1.5% (not gated on CD45). In the example above, the T cell (CD3+) content of the lysed whole blood start sample and non-lysed final isolated fraction is 33.0% and 95.0% (gated on CD45), respectively, or 33.0% and 94.9% (not gated on CD45), respectively. The starting frequency of T cells in the non-lysed whole blood start sample above is 0.059% (data not shown).