EasySep™ Direct Human NK Cell Isolation Kit

Immunomagnetic negative selection from whole blood kit
概要
The EasySep™ Direct Human NK Cell Isolation Kit is designed to isolate NK cells from human whole blood by immunomagnetic negative selection. The EasySep™ Direct procedure involves labeling RBCs, platelets and unwanted cells present in human whole blood with antibody complexes and magnetic particles. The magnetically labeled RBCs, platelets and cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Advantages
• > 99.9% red blood cell (RBC) depletion without the need for density gradient centrifugation, sedimentation, or lysis
• Up to 84% purity of isolated cells
• Fast, easy-to-use and column-free
• Isolated cells are untouched
Components
  • EasySep™ Direct Human NK Cell Isolation Kit (Catalog #19665)
    • EasySep™ Direct Human NK Cell Isolation Cocktail, 2 x 2.5 mL
    • EasySep™ Direct RapidSpheres™, 4 x 2.5 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
NK Cells
Species
Human
Sample Source
Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology
数据及文献

Data

Figure 1. Typical EasySep™ Direct Human NK Cell Isolation Profile

In the above example, the NK cell (CD3-CD56+) content of the lysed whole blood start sample and non-lysed final isolated fraction is 10.6% and 80.0% (gated on CD45), respectively, or 10.5% and 79.7% (not gated on CD45), respectively. The starting frequency of NK cells in the non-lysed whole blood start sample above is 0.011% (data not shown).

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